Involvement of Retinoid X Receptor in Imposex Development in Nucella lapillus and Nassarius reticulatus—Preliminary Results
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چکیده
The imposex phenomenon provides one of the best examples of endocrine disruption in wildlife and has been studied for about four decades. However, the exact biochemical mechanism by which it develops is still unclear. Several pathways have been proposed: neuroendocrine, steroid and retinoic. In the present work the possible role of retinoid X receptor (RXR) in the development of imposex in Nucella lapillus and Nassarius reticulatus was investigated: snails of both species were injected with ethanol containing tributyltin (TBT) or with fetal bovine serum (FBS) containing 9-cis-retinoic acid (9CRA: the natural ligand for humans RXRs) and maintained in a flowthrough system with artificial seawater for 30 days (temperature: 18.4 ± 0.44°C; salinity: 33.0 ± 1.06‰). Both TBT and 9CRA induced imposex development in N. lapillus and N. reticulatus. Significant (p < 0.05) increases in the vas deferens sequence index (VDSI) and female penis length (FPL) were registered between the ethanol control and the TBT treatment and between the FBS control and the 9CRA treatment. The obtained results provide further evidence of the involvement of RXR signalling pathway on imposex development in both species.
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تاریخ انتشار 2010